منابع مشابه
Assay of DNA-RNA hybrids by S1 nuclease digestion and adsorption to DEAE-cellulose filters.
A fast and accurate assay procedure for DNA-RNA hybrids is described in which exhaustive digestion of unhybridized DNA with S1 nuclease is followed by binding of hybrids to filter discs of DEAE-cellulose. The digested DNA can be efficiently washed from the filters so that background levels of 0.1-0.2% of input tracer DNA can be achieved, in contrast to the much higher (approximately 1-5%) backg...
متن کاملPurification of peptidyl-tRNA on benzoylated DEAE-cellulose columns.
The o-nitrophenylsulfenyl group, and amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.
متن کاملAdsorption of Nuclease P1 on Chitosan Nano-particles
The sorption of nuclease P1 onto chitosan nano-particles is studied in this paper. The effect of some adsorption kinetics factors such as nuclease P1 concentration, chitosan nano-particles solution concentration, adsorption temperature, chitosan nano-particles size, solution pH, etc. is investigated. Adsorption of nuclease P1 onto chitosan nano-particles is fitted into Lagergren first-order equ...
متن کاملImmobilization of Nuclease p1 on Chitosan Micro-spheres
L. E. Shi, Z. X. Tang, Y. Yi, J. S. Chen, W. Y. Xiong, and G. Q. Ying College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, Zhejiang, China College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China Department of Food Science and Technology, College of Biotechnology, East China University of Science and Technology....
متن کاملA DEAE-cellulose batch technique for antilymphyocyte globulin preparation.
Absorption: With the original technique (1) the horse serum decomplemented by heating to 56 0 C. and then absorbed against cells and pooled serum of the species to be treated; tion was done first to avoid hemolysis of the added red cedure had two flaws. First, the use of serum (rather than p.,,"~ ... aJ ICtJ,:'lgror absorption precluded the removal of antifibrinogen and bocyte antibodies. Secon...
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ژورنال
عنوان ژورنال: Brazilian Journal of Chemical Engineering
سال: 2010
ISSN: 0104-6632
DOI: 10.1590/s0104-66322010000100003